Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GFPT1 antibody [EPR4854] (ab125069)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4854] to GFPT1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GFPT1 antibody [EPR4854]
    See all GFPT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4854] to GFPT1

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GFPT1 aa 600-700. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human testis, and Human breast carcinoma tissue; WB: Human placenta lysate, MCF7, C6, MEF, 293T, JAR and HeLa cell line lysates, Mouse heart and Mouse cerebral cortex, Rat heart tissue lysate. IP: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (ab125069)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (ab125069)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling GFPT1 with Purified ab125069 at 1:1000 dilution (2.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-GFPT1 antibody [EPR4854] (ab125069)
    Immunoprecipitation - Anti-GFPT1 antibody [EPR4854] (ab125069)

    ab125069 (purified) at 1:100 dilution (2µg) immunoprecipitating GFPT1 in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab125069 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab125069 in Jurkat whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069)
    Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069)
    Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/200 dilution (Purified) + C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa

  • Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069)
    Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069)
    All lanes : Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution (Purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Mouse cerebral cortex lysates
    Lane 4 : MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates
    Lane 5 : Rat heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa



    This antibody is not suitable to detect tissue lysate samples.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (ab125069)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (ab125069)

    ab125069, at a 1/100 dilution, staining GFPT1 in paraffin-embedded Human testis tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069)
    Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069)
    All lanes : Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 79 kDa

  • Anti-GFPT1 antibody [EPR4854] (ab125069)
    Anti-GFPT1 antibody [EPR4854] (ab125069)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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