Anti-GFAP antibody [EPR1034Y] (ab279291)
![Anti-GFAP antibody [EPR1034Y] (ab279291)](https://www.abcam.com/ps/products/279/ab279291/Images/ab279291-450224-anti-gfap-antibody-epr1034yrtigg2a-western-blot-human-brain-tissue-lysate-20-g.jpg "Anti-GFAP antibody [EPR1034Y] (ab279291)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [EPR1034Y] to GFAP
- Suitable for: Flow Cyt (Intra), IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GFAP antibody [EPR1034Y]
See all GFAP primary antibodies -
Description
Rat monoclonal [EPR1034Y] to GFAP -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt (Intra) RatIHC-Fr MouseIHC-P HumanWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat brain lysate. IHC-P: Human cerebral cortex tissue. IHC-Fr: Mouse cerebrum tissue. Flow Cyt: Rat primary neural/glia cells.
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General notes
This rat antibody has been engineered from a RabMAb parent antibody (ab68428). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1034Y -
Isotype
IgG2 -
Research areas
Images
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Western blot - Anti-GFAP antibody (ab279291)All lanes : Anti-GFAP antibody [EPR1034Y] (ab279291) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilutionThe molecular weight observed is consistent with the literature (PMID: 824020, 2294, 6340792).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3.25 seconds; Lane 2, 3: 10 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y-RtIgG2a] (ab279291)](https://www.abcam.com/ps/products/279/ab279291/Images/ab279291-450189-anti-gfap-antibody-EPR1034Y-immunohistochemistry-cerebral-cortex-human-YR061602PS-RtIgG2a.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y-RtIgG2a] (ab279291)IHC image of GFAP staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab279291, 1µg/ml, for 15 mins at room temperature. A rabbit anti-rat IgG2a, ab102248, was added for 8 mins at room temperature and detected using an HRP conjugated goat anti-rabbit compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (Frozen sections) - Anti-GFAP antibody (ab279291)Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling GFAP with ab279291 at 1/500 (2.01 µg/ml) dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Positive staining on mouse cerebrum is observed.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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Flow Cytometry (Intracellular) - Anti-GFAP antibody (ab279291)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized rat primary neural/glia cells labelling GFAP with ab279291 at 1/1000 dilution (0.1µg)/ Right compared with a Rat monoclonal IgG isotype control/ Left.
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 647, ab150163) at 1/2000 dilution was used as the secondary antibody.
