Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1941Y] to Gelsolin plasma
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Gelsolin plasma antibody [EPR1941Y]
See all Gelsolin plasma primary antibodies -
Description
Rabbit monoclonal [EPR1941Y] to Gelsolin plasma -
Host species
Rabbit -
Specificity
The antibody only detects the isoform 1 of the protein (86kDa). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP MouseWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human plasma
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1941Y -
Isotype
IgG -
Research areas
Images
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Lanes 1-4 : Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/10000 dilution
Lanes 5-8 : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution
Lanes 9-12 : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution
Lanes 1 & 5 & 9 : THP-1 (human acute monocytic leukemia) whole cell lysate
Lanes 2 & 6 & 10 : THP-1 cultured medium
Lanes 3 & 7 & 11 : MCF-7 (human breast adenocarcinoma) whole cell lysate
Lanes 4 & 8 & 12 : Human Plasma
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 81/86 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBloking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBSTThe secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lane 5-8: ab109014 detected both secretory and cytoplasmic isoforms. Lane 9-12: ab134183 detected both secretory and cytoplasmic isoforms.
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Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/50000 dilution + human plasma at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
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ab75832 Immunoprecipitating Gelsolin plasma in mouse embryo whole cell lysate. 2µg of capture antibody (ab75832) per 0.35mg of lysate was used. 10µg of plasma was incubated with primary antibody (Panel A: ab75832, Panel B: ab134183) at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) ab131366 was used for detection at a dilution of 1/10000.
Lane 1: Human plasma
Lane 2: Human plasma
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.Exposure time: Panel A - 8 seconds, Panel B - 3 minutes.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The additional band at 50 kDa is a rabbit IgG heavy chain.
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ab75832 at 1/100 dilution staining Gelsolin plasma in human colon tissue, using a HRP/AP polymerized secondary antibody.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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