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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins

Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

Price and availability

304 886 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1941Y] to Gelsolin plasma
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Gelsolin plasma antibody [EPR1941Y]
    See all Gelsolin plasma primary antibodies
  • Description

    Rabbit monoclonal [EPR1941Y] to Gelsolin plasma
  • Host species

    Rabbit
  • Specificity

    The antibody only detects the isoform 1 of the protein (86kDa).
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Mouse
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human plasma
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1941Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly

Images

  • Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
    Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
    Lanes 1-4 : Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/10000 dilution
    Lanes 5-8 : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution
    Lanes 9-12 : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution

    Lanes 1 & 5 & 9 : THP-1 (human acute monocytic leukemia) whole cell lysate
    Lanes 2 & 6 & 10 : THP-1 cultured medium
    Lanes 3 & 7 & 11 : MCF-7 (human breast adenocarcinoma) whole cell lysate
    Lanes 4 & 8 & 12 : Human Plasma

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 81/86 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Bloking buffer 5% NFDM/TBST
    Diluting buffer 5% NFDM/TBST

    The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lane 5-8: ab109014 detected both secretory and cytoplasmic isoforms. Lane 9-12: ab134183 detected both secretory and cytoplasmic isoforms.

  • Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
    Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
    Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/50000 dilution + human plasma at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
    Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

    ab75832 Immunoprecipitating Gelsolin plasma in mouse embryo whole cell lysate. 2µg of capture antibody (ab75832) per 0.35mg of lysate was used. 10µg of plasma was incubated with primary antibody (Panel A: ab75832, Panel B: ab134183) at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) ab131366 was used for detection at a dilution of 1/10000.

    Lane 1: Human plasma
    Lane 2: Human plasma
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.

    Exposure time: Panel A - 8 seconds, Panel B - 3 minutes.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    The additional band at 50 kDa is a rabbit IgG heavy chain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

    ab75832 at 1/100 dilution staining Gelsolin plasma in human colon tissue, using a HRP/AP polymerized secondary antibody.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)
    Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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