Anti-GCKR antibody [OTI1E7] (ab279376)
![Anti-GCKR antibody [OTI1E7] (ab279376)](https://www.abcam.com/ps/products/279/ab279376/Images/ab279376-4-AntiGCKR-antibody-OTI1E7-immunohistochemistry-Carcinoma-kidney-tissue-human.jpg "Anti-GCKR antibody [OTI1E7] (ab279376)")
Key features and details
- Mouse monoclonal [OTI1E7] to GCKR
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GCKR antibody [OTI1E7]
See all GCKR primary antibodies -
Description
Mouse monoclonal [OTI1E7] to GCKR -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human GCKR aa 1-625. NP_001417
Database link: Q14397 -
Positive control
- WB: HeLa, A549, COS7, Jurkat cells. IHC-P: Carcinoma of human kidney tissue, kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
OTI1E7 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCKR antibody [OTI1E7] (ab279376)
Paraffin embedded carcinoma of human kidney tissue stained for GCKR using ab279376 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCKR antibody [OTI1E7] (ab279376)](https://www.abcam.com/ps/products/279/ab279376/Images/ab279376-3-AntiGCKR-antibody-OTI1E7-immunohistochemistry-Kidney-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCKR antibody [OTI1E7] (ab279376)Paraffin embedded human kidney tissue stained for GCKR using ab279376 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10minutes.
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![Western blot - Anti-GCKR antibody [OTI1E7] (ab279376)](https://www.abcam.com/ps/products/279/ab279376/Images/ab279376-2-AntiGCKR-antibody-OTI1E7-westernblot-HepG2-HeLa-SVT2-A549-COS7-Jurkat-MDCK-PC12-MCF7-cells.jpg)
Western blot - Anti-GCKR antibody [OTI1E7] (ab279376)All lanes : Anti-GCKR antibody [OTI1E7] (ab279376) at 1/2000 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS-7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 69 kDa
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![Western blot - Anti-GCKR antibody [OTI1E7] (ab279376)](https://www.abcam.com/ps/products/279/ab279376/Images/ab279376-1-AntiGCKR-antibody-OTI1E7-westernblot-HEK293Tcells.jpg)
Western blot - Anti-GCKR antibody [OTI1E7] (ab279376)All lanes : Anti-GCKR antibody [OTI1E7] (ab279376) at 1/2000 dilution
Lane 1 : HEK293T cells transfected with the pCMV6-ENTRY control
Lane 2 : HEK293T cells were transfected with the pCMV6-ENTRY GCKR cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 69 kDa