Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13450] to GCAT - BSA and Azide free
  • Suitable for: IP, IHC-P, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GCAT antibody [EPR13450] - BSA and Azide free
    See all GCAT primary antibodies

  • Description

    Rabbit monoclonal [EPR13450] to GCAT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250342 is the carrier-free version of ab181094.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    Western blot - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    All lanes : Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution

    Lane 1 : BxPC-3 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    This data was developed using ab181094, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    Western blot - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution + Human fetal liver tissue lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    This data was developed using ab181094, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    This data was developed using ab181094, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human heart tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    This data was developed using ab181094, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human pancreas tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    Immunocytochemistry - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    This data was developed using ab181094, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells, staining GCAT (green) with ab181094 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
  • Immunoprecipitation - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    Immunoprecipitation - Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)

    This data was developed using ab181094, the same antibody clone in a different buffer formulation.

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or negative control (lane 2), labeling GCAT immunoprecipitated using ab181094 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)
    Anti-GCAT antibody [EPR13450] - BSA and Azide free (ab250342)

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