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Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14889] to GBF1 - C-terminal
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GBF1 antibody [EPR14889] - C-terminal
    See all GBF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14889] to GBF1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, 293, PC12 and NIH 3T3 cell lysates; Human kidney and Mouse cardiac muscle tissues; 293 and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    All lanes : Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 206 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/50000 dilution + 293 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 206 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    All lanes : Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/5000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 206 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GBF1 with ab189512 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin (Inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling GBF1 with ab189512 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin (Inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Immunocytochemistry/ Immunofluorescence - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling GBF1 with ab189512 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).

  • Flow Cytometry - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Flow Cytometry - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GBF1 with ab189512 at 1/230 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)
    Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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