Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14889] to GBF1 - C-terminal
Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-GBF1 antibody [EPR14889] - C-terminal
See all GBF1 primary antibodies
Description

Rabbit monoclonal [EPR14889] to GBF1 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
WB	Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, Jurkat, 293, PC12 and NIH 3T3 cell lysates; Human kidney and Mouse cardiac muscle tissues; 293 and HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14889
Isotype

IgG
Research areas

Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

All lanes : Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/20000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 206 kDa
Observed band size: 250 kDa
why is the actual band size different from the predicted?
Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/50000 dilution + 293 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 206 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Western blot - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

All lanes : Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512) at 1/5000 dilution

Lane 1 : PC12 cell lysate
Lane 2 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 206 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GBF1 with ab189512 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin (Inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling GBF1 with ab189512 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin (Inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling GBF1 with ab189512 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).
Flow Cytometry - Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GBF1 with ab189512 at 1/230 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Anti-GBF1 antibody [EPR14889] - C-terminal (ab189512)

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