Anti-GBE1 antibody [EP11113] (ab180596)
![Anti-GBE1 antibody [EP11113] (ab180596)](https://www.abcam.com/ps/products/180/ab180596/Images/ab180596-211359-anti-gbe1-antibody-ep11113-western-blot.jpg "Anti-GBE1 antibody [EP11113] (ab180596)")
Key features and details
- Rabbit monoclonal [EP11113] to GBE1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GBE1 antibody [EP11113]
See all GBE1 primary antibodies -
Description
Rabbit monoclonal [EP11113] to GBE1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, PC-3 or Human skeletal muscle lysate, HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP11113 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GBE1 antibody [EP11113] (ab180596)All lanes : Anti-GBE1 antibody [EP11113] (ab180596) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : PC-3 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
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![Western blot - Anti-GBE1 antibody [EP11113] (ab180596)](https://www.abcam.com/ps/products/180/ab180596/Images/ab180596-211361-anti-gbe1-antibody-ep11113-western-blot.jpg)
Western blot - Anti-GBE1 antibody [EP11113] (ab180596)Anti-GBE1 antibody [EP11113] (ab180596) at 1/2000 dilution + Human skeletal muscle lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
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![Immunocytochemistry/ Immunofluorescence - Anti-GBE1 antibody [EP11113] (ab180596)](https://www.abcam.com/ps/products/180/ab180596/Images/ab180596-211362-anti-gbe1-antibody-ep11113-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GBE1 antibody [EP11113] (ab180596)Immunofluorescence analysis of HeLa cells (fixative 4% paraformaldehyde) labeling GBE1 with ab180596 at a 1/100 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.
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![Flow Cytometry - Anti-GBE1 antibody [EP11113] (ab180596)](https://www.abcam.com/ps/products/180/ab180596/Images/ab180596-211364-anti-gbe1-antibody-ep11113-flow-cytometry.jpg)
Flow Cytometry - Anti-GBE1 antibody [EP11113] (ab180596)Flow cytometry analysis of HeLa cells using ab180596 at a 1/40 dilution (red) and a rabbit IgG as negative control (green).
