Anti-GATA4 antibody (ab124265)
Key features and details
- Rabbit polyclonal to GATA4
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GATA4 antibody
See all GATA4 primary antibodies -
Description
Rabbit polyclonal to GATA4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 392-442 of Human GATA4 (NP_002043.2).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab124265 was affinity purified using an epitope specific to GATA4 immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody (ab124265)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling GATA4 with ab124265 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-GATA4 antibody (ab124265)All lanes : Anti-GATA4 antibody (ab124265) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg/ml
Lane 2 : 293T whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 50 µg/ml
Lane 4 : Jurkat whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-GATA4 antibody (ab124265)ab124265 at 1 µg/ml detecting GATA4 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of GATA4
Lane 2: ab124265 at 6 µg/mg of lysate
Lane 3: Control IgG
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds