Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12377(B)] to GANAB - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free
    See all GANAB primary antibodies

  • Description

    Rabbit monoclonal [EPR12377(B)] to GANAB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250111 is the carrier-free version of ab179805 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250111 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as alpha Glucosidase II

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
    Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)

    This data was developed using ab179805, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: GANAB knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4:  cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab179805 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab179805 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab179805 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
    Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
    This data was developed using ab179805, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from A431 cell lysate using ab179805.
  • Flow Cytometry - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
    Flow Cytometry - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
    This data was developed using ab179805, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells labeling GANAB with ab179805 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
  • Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
    Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
    All lanes : Anti-GANAB antibody [EPR12377(B)] (ab179805) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : A431 lysate
    Lane 3 : HepG2 lysate
    Lane 4 : HeLa lysate
    Lane 5 : PC-12 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 107 kDa



    This data was developed using ab179805, the same antibody clone in a different buffer formulation.

     

  • Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
    Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)

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