Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23853-132] to galectin 9/Gal-9
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-galectin 9/Gal-9 antibody [EPR23853-132]
    See all galectin 9/Gal-9 primary antibodies

  • Description

    Rabbit monoclonal [EPR23853-132] to galectin 9/Gal-9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus, liver and lung tissue lysates; EL4.IL-2, RAW 264.7 and NIH/3T3 whole cell lysates; Recombinant mouse galectin 9/Gal-9 protein. ICC: EL4.IL-2 cells. Flow Cyt: EL4.IL-2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
    Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
    All lanes : Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877) at 1/1000 dilution

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Mouse lung tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : EL4.IL-2 (mouse lymphoma t lymphocyte) whole cell lysate
    Lane 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 7 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    Lanes 1-4 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
    Lanes 5-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 40 kDa
    Observed band size: 15,36,40 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Bands of 40KDa and 36KDa respectively represent two different isoforms.

    Band of 15KDa may represents the cleaved form.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9038233, 11886844, 22805533, 15811318).

    Low expression: mouse brain (PMID: 9038233).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: Lanes 1-2: 5.5 seconds; Lanes 3-6: 37 seconds; Lanes 7: 3 minutes.

     

  • Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
    Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL-2 cells labelling galectin 9/Gal-9 with ab275877 at 1/50 (12.28 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in EL4.IL-2 cell line. The staining pattern observed is consistent with what has been described in the literature (PMID: 28750861). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
    Flow Cytometry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) cells labelling galectin 9/Gal-9 with ab275877 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
    Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
    Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877) at 1/1000 dilution + Recombinant mouse galectin 9/Gal-9 protein, 20 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

     

  • Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
    Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

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