Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19182] to galectin 9/Gal-9 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free
    See all galectin 9/Gal-9 primary antibodies

  • Description

    Rabbit monoclonal [EPR19182] to galectin 9/Gal-9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250745 is the carrier-free version of ab184331. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250745 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)
    Western blot - Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)
    All lanes : Anti-galectin 9/Gal-9 antibody [EPR19182] (ab184331) at 1/2000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse thymus lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 5 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab184331, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 15 seconds; Lanes 4-5: 3 minutes.

    galectin 9 / Gal-9 was not detected in mouse brain under our testing conditions, but mouse brain should have low expression of galectin 9 / Gal-9 according to the literature (PMID: 9038233). The molecular weight observed is consistent with what has been described in the literature (PMID: 9038233).

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)
    Western blot - Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)
    All lanes : Anti-galectin 9/Gal-9 antibody [EPR19182] (ab184331) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab184331, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)
    Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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