Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)
![Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)](https://www.abcam.com/ps/products/250/ab250745/Images/ab250745-403517-anti-galectin-9-gal-9-antibody-epr19182-bsa-and-azide-free-western-blot.jpg "Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19182] to galectin 9/Gal-9 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free
See all galectin 9/Gal-9 primary antibodies -
Description
Rabbit monoclonal [EPR19182] to galectin 9/Gal-9 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250745 is the carrier-free version of ab184331. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250745 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19182 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)All lanes : Anti-galectin 9/Gal-9 antibody [EPR19182] (ab184331) at 1/2000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse spleen lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?This data was developed using ab184331, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 15 seconds; Lanes 4-5: 3 minutes.
galectin 9 / Gal-9 was not detected in mouse brain under our testing conditions, but mouse brain should have low expression of galectin 9 / Gal-9 according to the literature (PMID: 9038233). The molecular weight observed is consistent with what has been described in the literature (PMID: 9038233).
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![Western blot - Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)](https://www.abcam.com/ps/products/250/ab250745/Images/ab250745-398252-anti-galectin-9-gal-9-antibody-epr19182-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)All lanes : Anti-galectin 9/Gal-9 antibody [EPR19182] (ab184331) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab184331, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)](https://www.abcam.com/ps/products/250/ab250745/Images/ab250745-3-benefits-of-recombinant-antibodies.png)
Anti-galectin 9/Gal-9 antibody [EPR19182] - BSA and Azide free (ab250745)
