Anti-GAD1 antibody (ab228710)
Key features and details
- Rabbit polyclonal to GAD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GAD1 antibody
See all GAD1 primary antibodies -
Description
Rabbit polyclonal to GAD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human GAD1 (internal sequence). The exact sequence is proprietary.
Database link: Q99259 -
Positive control
- WB: Mouse and rat brain whole tissue extracts; HEK-293T whole cell lysate. ICC/IF: DIV9 rat E18 primary cortical neurons. IHC/P: Adult mouse retina.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody (ab228710)
Paraffin-embedded adult mouse retina stained for GAD1 protein (green) using ab228710 at 1/250 dilution in immunohistochemical analysis.
Beta Tubulin 3/TUJ1 (red) stained by beta Tubulin 3/TUJ1 antibody diluted at 1/250.
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Western blot - Anti-GAD1 antibody (ab228710)All lanes : Anti-GAD1 antibody (ab228710) at 1/500 dilution
Lane 1 : Mouse brain whole tissue extract
Lane 2 : Rat brain whole tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 67 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-GAD1 antibody (ab228710)All lanes : Anti-GAD1 antibody (ab228710) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : GAD1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 67 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-GAD1 antibody (ab228710)DIV9 rat E18 primary cortical neurons stained for GAD1 (green) using ab228710 at 1/500 dilution in ICC/IF. Neurons were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Fluoroshield with DAPI (blue).
