Anti-GAB2 antibody [EP369Y] - BSA and Azide free (ab247248)
![Anti-GAB2 antibody [EP369Y] - BSA and Azide free (ab247248)](https://www.abcam.com/ps/products/247/ab247248/Images/ab247248-405385-anti-gab2-antibody-ep369y-bsa-and-azide-free-western-blot-k-562-human.jpg "Anti-GAB2 antibody [EP369Y] - BSA and Azide free (ab247248)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP369Y] to GAB2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GAB2 antibody [EP369Y] - BSA and Azide free
See all GAB2 primary antibodies -
Description
Rabbit monoclonal [EP369Y] to GAB2 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody is predicted to detect the splice isoform 2 based on sequence homology.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247248 is the carrier-free version of ab32365. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247248 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Whole Cell Lysate -
Clonality
Monoclonal -
Clone number
EP369Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAB2 antibody [EP369Y] - BSA and Azide free (ab247248)All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in RIPA lysis method
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in 1% SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?This data was developed using ab32365, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
We recommend to use 1% SDS Hot lysis prepare method to get desired western Blot results.
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![Anti-GAB2 antibody [EP369Y] - BSA and Azide free (ab247248)](https://www.abcam.com/ps/products/247/ab247248/Images/ab247248-2-benefits-of-recombinant-antibodies.png)
Anti-GAB2 antibody [EP369Y] - BSA and Azide free (ab247248)
