Anti-G-6-Pase antibody (ab93857)
Key features and details
- Rabbit polyclonal to G-6-Pase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G-6-Pase antibody
See all G-6-Pase primary antibodies -
Description
Rabbit polyclonal to G-6-Pase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cat, Macaque monkey
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and Hek293 whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).Target
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Function
Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Involvement in disease
Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A) [MIM:232200]. A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. -
Sequence similarities
Belongs to the glucose-6-phosphatase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2538 Human
- SwissProt: P35575 Human
- Unigene: 212293 Human
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Alternative names
- AW107337 antibody
- G-6-Pase antibody
- G6Pase antibody
see all
Images
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Western blot - Anti-G-6-Pase antibody (ab93857)All lanes : Anti-G-6-Pase antibody (ab93857) at 1 µg/ml
All lanes :
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Blocking buffer: 3% milk
Gel type: MOPS
Exposure Time: 12 minutes
Datasheets and documents
References (1)
ab93857 has been referenced in 1 publication.
- Karas K et al. The Dichotomous Nature of AZ5104 (an EGFR Inhibitor) Towards ROR? and ROR?T. Int J Mol Sci 20:N/A (2019). PubMed: 31744223
Images
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Western blot - Anti-G-6-Pase antibody (ab93857)All lanes : Anti-G-6-Pase antibody (ab93857) at 1 µg/ml
All lanes :
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Blocking buffer: 3% milk
Gel type: MOPS
Exposure Time: 12 minutes
