Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2546Y] to FYB - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-FYB antibody [EP2546Y] - BSA and Azide free
    See all FYB primary antibodies

  • Description

    Rabbit monoclonal [EP2546Y] to FYB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: TF-1 cells

  • General notes

    ab247435 is the carrier-free version of ab76103 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247435 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)
    Western blot - Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)
    Anti-FYB antibody [EP2546Y] (ab76103) at 1/10000 dilution + TF-1 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 85 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76103, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)
    This data was developed using ab76103, the same antibody clone in a different buffer formulation.ab76103, at 1/250 dilution, staining FYB in paraffin-embedded human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)
    Flow Cytometry - Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)
    This data was developed using ab76103, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of TF-1 (Human Erythroleukemia erythroblast) cells labeling FYB with purified ab76103 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)
    Anti-FYB antibody [EP2546Y] - BSA and Azide free (ab247435)

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