Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)
![Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)](https://www.abcam.com/ps/products/250/ab250467/Images/ab250467-405883-anti-fxyd6-plp-antibody-epr9004-2-bsa-and-azide-free-western-blot.jpg "Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9004(2)] to FXYD6/Plp - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free
See all FXYD6/Plp primary antibodies -
Description
Rabbit monoclonal [EPR9004(2)] to FXYD6/Plp - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250467 is the carrier-free version of ab181254 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250467 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as FXYD6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9004(2) -
Isotype
IgG
Images
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Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) at 1/50000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab181254, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)](https://www.abcam.com/ps/products/250/ab250467/Images/ab250467-402760-anti-fxyd6-plp-antibody-epr9004-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) at 1/10000 dilution + Human testis lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab181254, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)](https://www.abcam.com/ps/products/250/ab250467/Images/ab250467-399624-anti-fxyd6-plp-antibody-epr9004-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)This data was developed using ab181254, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation from Human testis lysate labeling FXYD6/Plp using ab181254 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1000 dilution.
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![Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)](https://www.abcam.com/ps/products/250/ab250467/Images/ab250467-4-benefits-of-recombinant-antibodies.png)
Anti-FXYD6/Plp antibody [EPR9004(2)] - BSA and Azide free (ab250467)