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Signal Transduction Metabolism Plasma Membrane ATPases

Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)

Price and availability

606 422 ₸

Availability

Order now and get it on Thursday June 01, 2023

Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16082] to FXYD1/PLM - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free
    See all FXYD1/PLM primary antibodies
  • Description

    Rabbit monoclonal [EPR16082] to FXYD1/PLM - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251304 is the carrier-free version of ab200204.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR16082
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics

Images

  • Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
    Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
    Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab200204, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
    Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
    All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/10000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab200204, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
    Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
    All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab200204, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
    Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)

    This data was developed using ab200204, the same antibody clone in a different buffer formulation.

    FXYD1/PLM was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal heart whole cell lysate 10ug (Input).

     Lane 2: ab200204 IP in Human fetal heart whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
    Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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