Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)
![Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)](https://www.abcam.com/ps/products/251/ab251304/Images/ab251304-408177-anti-fxyd1-plm-antibody-epr16082-bsa-and-azide-free-western-blot.jpg "Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16082] to FXYD1/PLM - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free
See all FXYD1/PLM primary antibodies -
Description
Rabbit monoclonal [EPR16082] to FXYD1/PLM - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251304 is the carrier-free version of ab200204.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16082 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab200204, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)](https://www.abcam.com/ps/products/251/ab251304/Images/ab251304-405865-anti-fxyd1-plm-antibody-epr16082-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/10000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab200204, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)](https://www.abcam.com/ps/products/251/ab251304/Images/ab251304-403285-anti-fxyd1-plm-antibody-epr16082-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)All lanes : Anti-FXYD1/PLM antibody [EPR16082] (ab200204) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab200204, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)](https://www.abcam.com/ps/products/251/ab251304/Images/ab251304-399574-anti-fxyd1-plm-antibody-epr16082-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)This data was developed using ab200204, the same antibody clone in a different buffer formulation.
FXYD1/PLM was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal heart whole cell lysate 10ug (Input).
Lane 2: ab200204 IP in Human fetal heart whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)](https://www.abcam.com/ps/products/251/ab251304/Images/ab251304-5-benefits-of-recombinant-antibodies.png)
Anti-FXYD1/PLM antibody [EPR16082] - BSA and Azide free (ab251304)