Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24439-141] to FSH beta - BSA and Azide free
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free
    See all FSH beta primary antibodies

  • Description

    Rabbit monoclonal [EPR24439-141] to FSH beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human pituitary, Mouse pituitary, Rat pituitary lysates. IHC-P: Human pituitary, Mouse pituitary, Rat pituitary tissues. IHC-Fr: Rat pituitary tissue.

  • General notes

    ab281570 is the carrier-free version of ab281562. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281570 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24439-141

  • Isotype

    IgG

Images

  • Western blot - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Western blot - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    All lanes : Anti-FSH beta antibody [EPR24439-141] (ab281562) at 1/1000 dilution

    Lane 1 : Human pituitary tissue lysate
    Lane 2 : Human heart tissue lysate
    Lane 3 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 15 kDa
    Observed band size: 23,19 kDa why is the actual band size different from the predicted?



    This data was developed using ab281562, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 29090093, 11502795).

    Negative control: heart, spleen (PMID:29090093).

     Exposure time: 81 seconds

     

     

  • Western blot - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Western blot - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    All lanes : Anti-FSH beta antibody [EPR24439-141] (ab281562) at 1/1000 dilution

    Lane 1 : Mouse pituitary tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat pituitary tissue lysate
    Lane 5 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 15 kDa
    Observed band size: 23,19 kDa why is the actual band size different from the predicted?



    This data was developed using ab281562, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 29090093, 11502795).

    Negative control: heart, spleen (PMID:29090093).

     Exposure time: Lanes 1-3:3 minutes; Lanes 4-5:26 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)

    This data was developed using ab281562, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pituitary tissue labelling FSH beta with ab281562 at 1/2000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human pituitary (PMID: 8930609). The section was incubated with ab281562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)

    This data was developed using ab281562, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse pituitary tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse pituitary. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)

    This data was developed using ab281562, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat pituitary tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat pituitary. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Immunohistochemistry (Frozen sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)

    This data was developed using ab281562, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pituitary tissue labeling FSH beta with ab281562 at 1/100 dilution (4.75 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat pituitary is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)

    This data was developed using ab281562, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human spleen. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)

    This data was developed using ab281562, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cardiac muscle. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)
    Anti-FSH beta antibody [EPR24439-141] - BSA and Azide free (ab281570)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.