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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box

Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)

Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2150(3)] to FOXO4/AFX - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free
    See all FOXO4/AFX primary antibodies
  • Description

    Rabbit monoclonal [EPR2150(3)] to FOXO4/AFX - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248170 is the carrier-free version of ab126757 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248170 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Epitomics, the University of Utrecht, Netherlands and the lab of Tobias Dansen. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.81 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2150(3)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXO

Images

  • Western blot - Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)
    Western blot - Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)
    All lanes : Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757) at 1/10000 dilution

    Lane 1 : HA-FOXO4/AFX transfected 293T cell lysate
    Lane 2 : HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate
    Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab126757, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)
    This data was developed using ab126757, the same antibody clone in a different buffer formulation.ab126757, at 1/100 dilution, staining FOXO4/AFX in paraffin-embedded Human placenta tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)
    OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)
    This data was developed using ab126757, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)
    Anti-FOXO4/AFX antibody [EPR2150(3)] - BSA and Azide free (ab248170)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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