Anti-FOXA2 antibody [EPR4465] - BSA and Azide free (ab247656)
![Anti-FOXA2 antibody [EPR4465] - BSA and Azide free (ab247656)](https://www.abcam.com/ps/products/247/ab247656/Images/ab247656-1-anti-foxa2-antibody-epr4465-62-7-chromatin-immunoprecipitation-hepg2-human.jpg "Anti-FOXA2 antibody [EPR4465] - BSA and Azide free (ab247656)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4465] to FOXA2 - BSA and Azide free
- Suitable for: ICC/IF, ChIP, WB
- Reacts with: Human
Overview
-
Product name
Anti-FOXA2 antibody [EPR4465] - BSA and Azide free
See all FOXA2 primary antibodies -
Description
Rabbit monoclonal [EPR4465] to FOXA2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab247656 is the carrier-free version of ab108396. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247656 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4465 -
Isotype
IgG -
Research areas
Images
-
ChIP - Anti-FOXA2 antibody [EPR4465] - BSA and Azide free (ab247656)
Chromatin was prepared from HepG2 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab108396 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are located in the first kb of the transcribed region.
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108396). -
![Anti-FOXA2 antibody [EPR4465] - BSA and Azide free (ab247656)](https://www.abcam.com/ps/products/247/ab247656/Images/ab247656-2-benefits-of-recombinant-antibodies.png)
Anti-FOXA2 antibody [EPR4465] - BSA and Azide free (ab247656)
