Anti-Follistatin antibody (ab64490)
Key features and details
- Rabbit polyclonal to Follistatin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Follistatin antibody
See all Follistatin primary antibodies -
Description
Rabbit polyclonal to Follistatin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Follistatin.
Read Abcam's proprietary immunogen policy (Peptide available as ab74741.) -
Positive control
- Purchase matching WB positive control:Recombinant Human Follistatin protein
- Recombinant human Follistatin protein (ab50163) can be used as a positive control in WB. This antibody gave a positive signal in the following Lysates: U2OS Whole Cell, Mouse Liver Tissue, Raji Whole Cell, MOLT4 Whole Cell, Mouse Colon Tissue, Rat Liver Tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab64490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Target
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Function
Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). -
Tissue specificity
Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid. -
Sequence similarities
Contains 3 follistatin-like domains.
Contains 3 Kazal-like domains.
Contains 1 TB (TGF-beta binding) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 396119 Chicken
- Entrez Gene: 327681 Cow
- Entrez Gene: 10468 Human
- Entrez Gene: 14313 Mouse
- Entrez Gene: 445002 Pig
- Entrez Gene: 24373 Rat
- Omim: 136470 Human
- SwissProt: Q90844 Chicken
see all -
Alternative names
- Activin binding protein antibody
- Activin-binding protein antibody
- Follistatin antibody
see all
Images
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Western blot - Anti-Follistatin antibody (ab64490)All lanes : Anti-Follistatin antibody (ab64490) at 1 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 5 : Colon (Mouse) Tissue Lysate
Lane 6 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 15 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
References (4)
ab64490 has been referenced in 4 publications.
- Liu N et al. Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling. PLoS Genet 15:e1008368 (2019). PubMed: 31518356
- Singh R et al. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes 11:65-84 (2018). PubMed: 29618935
- Fitzgerald AM et al. The effects of transforming growth factor-ß2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues. Invest Ophthalmol Vis Sci 53:7358-69 (2012). WB . PubMed: 23010638
- Garcia LA et al. 1,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells. Endocrinology 152:2976-86 (2011). WB ; Mouse . PubMed: 21673099
Images
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Western blot - Anti-Follistatin antibody (ab64490)All lanes : Anti-Follistatin antibody (ab64490) at 1 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 5 : Colon (Mouse) Tissue Lysate
Lane 6 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 15 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
