Anti-FMO5 antibody [EPR14783] (ab189516)
![Anti-FMO5 antibody [EPR14783] (ab189516)](https://www.abcam.com/ps/products/189/ab189516/Images/ab189516-225158-anti-fmo5-antibody-epr14783-western-blot.jpg "Anti-FMO5 antibody [EPR14783] (ab189516)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14783] to FMO5
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-FMO5 antibody [EPR14783]
See all FMO5 primary antibodies -
Description
Rabbit monoclonal [EPR14783] to FMO5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver tissue lysate and HepG2 cell lysate. IHC-P: Human liver tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14783 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FMO5 antibody [EPR14783] (ab189516)Anti-FMO5 antibody [EPR14783] (ab189516) at 1/10000 dilution + Human fetal liver at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
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![Western blot - Anti-FMO5 antibody [EPR14783] (ab189516)](https://www.abcam.com/ps/products/189/ab189516/Images/ab189516-225156-anti-fmo5-antibody-epr14783-western-blot.jpg)
Western blot - Anti-FMO5 antibody [EPR14783] (ab189516)Anti-FMO5 antibody [EPR14783] (ab189516) at 1/10000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO5 antibody [EPR14783] (ab189516)](https://www.abcam.com/ps/products/189/ab189516/Images/ab189516-225155-anti-fmo5-antibody-epr14783-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FMO5 antibody [EPR14783] (ab189516)Immunohistochemical analysis of paraffine embedded Human liver tissue labeling FMO5 with ab189516 at 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-FMO5 antibody [EPR14783] (ab189516)](https://www.abcam.com/ps/products/189/ab189516/Images/ab189516-225153-anti-fmo5-antibody-epr14783-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FMO5 antibody [EPR14783] (ab189516)Immunoprecipitation of Human fetal liver labeling FMO5 with ab189516 at 1/50 diltion and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 (+) or PBS (-)
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![Anti-FMO5 antibody [EPR14783] (ab189516)](https://www.abcam.com/ps/products/189/ab189516/Images/ab189516-6-benefits-of-recombinant-antibodies.png)
Anti-FMO5 antibody [EPR14783] (ab189516)
