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Immunology Adaptive Immunity T Cells CD

Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free (ab245824)

Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free (ab245824)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22232-318] to Flt3 / CD135 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free
    See all Flt3 / CD135 primary antibodies
  • Description

    Rabbit monoclonal [EPR22232-318] to Flt3 / CD135 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: THP-1 whole cell lysate.
  • General notes

    Ab245824 is the carrier-free version of ab245116. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245824 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22232-318
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases

Images

  • Immunoprecipitation - Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free (ab245824)
    Immunoprecipitation - Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free (ab245824)

    Flt3 / CD135 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line) whole cell lysate with ab245116 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245116 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: THP-1 whole cell lysate 10 μg (Input).
    Lane 2: ab245116 IP in THP-1 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245116 in THP-1 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 minute.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245116).

     

  • Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free (ab245824)
    Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free (ab245824)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Flt3 / CD135 antibody [EPR22232-318] - BSA and Azide free (ab245824)

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    Anti-Flt3 / CD135 antibody (ab197838)

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