Anti-Flotillin 1 antibody - C-terminal (ab228585)
Key features and details
- Rabbit polyclonal to Flotillin 1 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Flotillin 1 antibody - C-terminal
See all Flotillin 1 primary antibodies -
Description
Rabbit polyclonal to Flotillin 1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human Flotillin 1 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: O75955 -
Positive control
- WB: HepG2 whole cell lysate. IP: HCT-116 whole cell extract.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Flotillin 1 antibody - C-terminal (ab228585)Anti-Flotillin 1 antibody - C-terminal (ab228585) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 47 kDa10% SDS-PAGE
-
Immunoprecipitation - Anti-Flotillin 1 antibody - C-terminal (ab228585)Flotillin 1 was immunoprecipitated from HCT-116 (human colorectal carcinoma cell line) whole cell extract with 4 μg ab228585. Western blot was performed from the immunoprecipitate using ab228585 at 1/1000 dilution.
Lane 1: Control pre-immune IgG IP in HCT-116 whole cell extract.
Lane 2: ab228585 IP in HCT-116 whole cell extract.
10% SDS-PAGE
