Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FHL2 antibody [EPR17860-23] - BSA and Azide free (ab251380)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17860-23] to FHL2 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FHL2 antibody [EPR17860-23] - BSA and Azide free
    See all FHL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17860-23] to FHL2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-2 OS, HeLa and K562 cell lysates.

  • General notes

    Ab251380 is the carrier-free version of ab202586. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251380 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FHL2 antibody [EPR17860-23] - BSA and Azide free (ab251380)
    Western blot - Anti-FHL2 antibody [EPR17860-23] - BSA and Azide free (ab251380)
    All lanes : Anti-FHL2 antibody [EPR17860-23] (ab202586) at 1/1000 dilution

    Lane 1 : Wild-type U-2 OS cell lysate
    Lane 2 : FHL2 knockout U-2 OS cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab202586).

    Lanes 1 - 3: Merged signal (red and green). Green - ab202586 observed at 32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab202586 was shown to react with FHL2 in wild-type U-2 OS cells in western blot with loss of signal observed in FHL2 knockout sample. Wild-type and FHL2 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab202586 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-FHL2 antibody [EPR17860-23] - BSA and Azide free (ab251380)
    Western blot - Anti-FHL2 antibody [EPR17860-23] - BSA and Azide free (ab251380)
    All lanes : Anti-FHL2 antibody [EPR17860-23] (ab202586) at 1/1000 dilution

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : FHL2 knockout HeLa lysate
    Lane 3 : K562 lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 32 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab202586).

    Lanes 1-3: Merged signal (red and green). Green - ab202586 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab202586 Recombinant Anti-FHL2 antibody [EPR17860-23] was shown to specifically react with FHL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265475 (knockout cell lysate ab257441) was used. Wild-type and FHL2 knockout samples were subjected to SDS-PAGE. ab202586 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Anti-FHL2 antibody [EPR17860-23] - BSA and Azide free (ab251380)
    Anti-FHL2 antibody [EPR17860-23] - BSA and Azide free (ab251380)

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