Anti-FGD1 antibody (ab226473)
Key features and details
- Rabbit polyclonal to FGD1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FGD1 antibody
See all FGD1 primary antibodies -
Description
Rabbit polyclonal to FGD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human FGD1 aa 50-100. The exact sequence is proprietary.
Database link: P98174 -
Positive control
- IP: HeLa cell lysate.
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General notes
ab226473 has not performed satisfactorily when used for WB of FGD1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of FGD1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-FGD1 antibody (ab226473)
FGD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1.0 mg for IP, 20% of IP loaded) with ab226473 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226473 at 1 µg/ml.
Lane 1: ab226473 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
