Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FDPS/FPS antibody [005] (ab277132)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [005] to FDPS/FPS
  • Suitable for: IP, WB, ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-FDPS/FPS antibody [005]
    See all FDPS/FPS primary antibodies

  • Description

    Rabbit monoclonal [005] to FDPS/FPS

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human FDPS/FPS aa 1-353. Genbank: NP_001129294.1
    Database link: P14324

  • Positive control

    • Flow cyt: HeLa cells. ICC: HUVEC cells. IP: HeLa, HepG2 and A549 whole cell lysates. WB: HepG2 and HeLa whole cell lysates.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-FDPS/FPS antibody [005] (ab277132)
    Flow Cytometry - Anti-FDPS/FPS antibody [005] (ab277132)

    Flow cytometric analysis of FDPS/FPS expression on HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were stained with ab277132 at 1/25 dilution, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  • Immunoprecipitation - Anti-FDPS/FPS antibody (ab277132)
    Immunoprecipitation - Anti-FDPS/FPS antibody (ab277132)

    FDPS/FPS was immunoprecipitated from:

    Lane A:0.5 mg Hela (human epithelial cell line from cervix adenocarcinoma) Whole Cell Lysate
    Lane B:0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) Whole Cell Lysate
    Lane C:0.5 mg A549 (human lung carcinoma cell line) Whole Cell Lysate

    2 µL ab277132 and 15 µl of 50 % Protein G agarose.

    Western blot was performed using ab277132, at 1/100 dilution

    Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution

    Performed under reducing conditions.

  • Western blot - Anti-FDPS/FPS antibody (ab277132)
    Western blot - Anti-FDPS/FPS antibody (ab277132)
    All lanes : Anti-FDPS/FPS antibody [005] (ab277132) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 48 kDa



    Performed under reducing conditions.

  • Immunocytochemistry - Anti-FDPS/FPS antibody (ab277132)
    Immunocytochemistry - Anti-FDPS/FPS antibody (ab277132)

    Immunofluorescence staining of FDPS/FPS in HUVEC (human umbilical vein endothelial cell line) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab277132 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with an Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). 

  • Anti-FDPS/FPS antibody [005] (ab277132)
    Anti-FDPS/FPS antibody [005] (ab277132)

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