Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

500 µl 311 587 ₸

Order now and get it on Monday March 15, 2021

Anti-Factor XIIIa antibody [AC-1A1] (ab1834)

Key features and details

Mouse monoclonal [AC-1A1] to Factor XIIIa
Suitable for: IHC-P, WB, Flow Cyt
Reacts with: Human
Isotype: IgG1

Product name

Anti-Factor XIIIa antibody [AC-1A1]
See all Factor XIIIa primary antibodies
Description

Mouse monoclonal [AC-1A1] to Factor XIIIa
Host species

Mouse
Tested applications

Suitable for: IHC-P, WB, Flow Cytmore details
Species reactivity

Reacts with: Human
Immunogen

BALB/C mice were injected with recombinant human protein corresponding to A-subunit of coagulation Factor XIII.
Positive control
- Placenta
General notes

This antibody recognizes both the dimer and monomer forms.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Purity

IgG fraction
Clonality

Monoclonal
Clone number

AC-1A1
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)

ab1834 - immunohistochemistry

Formalin fixed paraffin embedded human placenta stained with Factor XIIIa, using ABC and AEC chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)

ab1834 staining human placenta by IHC-P.
Western blot - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)

Anti-Factor XIIIa antibody [AC-1A1] (ab1834) at 1/250 dilution + Human placenta tissue lysate - total protein at 10 µg

Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 37 kDa, 74 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 2 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Factor XIIIa with ab1834.
Flow Cytometry - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)

Overlay histogram showing A549 cells stained with ab16956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16956, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

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