Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Factor VIII antibody [EPR24039-262] (ab275376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24039-262] to Factor VIII
  • Suitable for: WB, IHC-P, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Factor VIII antibody [EPR24039-262]
    See all Factor VIII primary antibodies

  • Description

    Rabbit monoclonal [EPR24039-262] to Factor VIII

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma; HUVEC whole cell lysate. IHC-P: Human colon carcinoma and bladder cancer tissue; Mouse spleen and lung tissue; Rat lung tissue. ICC: HUVEC cells. Flow cyt: HUVEC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24039-262

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the blood vessels in human colon carcinoma (PMID: 18846440). The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Flow Cytometry - Anti-Factor VIII antibody [EPR24039-262] (ab275376)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293 (Human embryonic kidney epithelial cell line, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling Factor VIII with ab275376 at 1/500 dilution. 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK-293 (PMID: 31727959). HUVEC cells expressing Factor VIII is reported in literature (PMID: 30814851).

  • Immunocytochemistry - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Immunocytochemistry - Anti-Factor VIII antibody [EPR24039-262] (ab275376)

    Immunofluorescent analysis of 100% methanol-fixed HUVEC cells labelling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HUVEC cell line. Negative control: HEK-293 (PMID: 31727959). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Western blot - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Anti-Factor VIII antibody [EPR24039-262] (ab275376) at 1/1000 dilution + Human plasma at 20 µl

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 267 kDa
    Observed band size: 210-280,330 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 3085106).

    Exposure time: 10 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat lung (PMID: 25911555). The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Western blot - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    All lanes : Anti-Factor VIII antibody [EPR24039-262] (ab275376) at 1/1000 dilution

    Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 267 kDa
    Observed band size: 166,210-280,330 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HEK-293 (PMID: 27208572).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 3085106).

    Exposure time: 26 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse lung (PMID: 25911555). The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the megakaryocytes and platelets in mouse spleen (PMID: 25911555). The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VIII antibody [EPR24039-262] (ab275376)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Factor VIII with ab275376 at 1/1000 (0.457 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the blood vessels in human bladder cancer. The section was incubated with ab275376 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Factor VIII antibody [EPR24039-262] (ab275376)
    Anti-Factor VIII antibody [EPR24039-262] (ab275376)

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