Anti-Factor B antibody (ab72658)
Key features and details
- Rabbit polyclonal to Factor B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Factor B antibody
See all Factor B primary antibodies -
Description
Rabbit polyclonal to Factor B -
Host species
Rabbit -
Specificity
ab72658 detects a fragment from activated Factor B; the Bb fragment, resulting from cleavage adjacent to Lys260. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
KIVLDPSGSMN IYLVLDGSD
, corresponding to amino acids 260-280 of Human Factor B. -
Positive control
- Extracts from K562 cells, treated with etoposide (25uM, 1hour).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Factor B antibody (ab72658)All lanes : Anti-Factor B antibody (ab72658) at 1/200 dilution
Lane 1 : Extracts from K562 cells, treated with etoposide (25uM, 1hour)
Lane 2 : Extracts from K562 cells, treated with etoposide (25uM, 1hour) with Cl-peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 54 and 86 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding), 75 kDa. We are unsure as to the identity of these extra bands.
