Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)
![Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)](https://www.abcam.com/ps/products/90/ab90247/Images/ab90247-258566-anti-f4-80-antibody-f4-80-immunofluorescence.jpg "Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)")
Key features and details
- Rat monoclonal [F4/80] to F4/80 - Macrophage Marker
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Overview
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Product name
Anti-F4/80 antibody [F4/80] - Macrophage Marker
See all F4/80 primary antibodies -
Description
Rat monoclonal [F4/80] to F4/80 - Macrophage Marker -
Host species
Rat -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
corresponding to F4/80.
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Positive control
- ICC/IF: Raw 264.7 cells.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
F4/80 -
Myeloma
NS1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)
ab90247 stained Raw 264.7 cells. The cells were 100% methanol fixed (5 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Triton for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90247 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
