Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23353-55] to Ezrin - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free
    See all Ezrin primary antibodies

  • Description

    Rabbit monoclonal [EPR23353-55] to Ezrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and A549 cell lysate; Human kidney tissue lysate; Mouse hippocampus and fetal brain tissue; Rat brain lysate; Neuro-2a, C6, PC-12 and NIH/3T3 cell lysate. IHC-P: Mouse kidney and spleen tissue; Rat kidney, liver and spleen tissue. ICC/IF: HeLa and Neuro-2a cells. Flow: HeLa, C6 and Neuro-2a cells. IP: HeLa, C6 and Neuro-2a cell lysate.

  • General notes

    ab270525 is the carrier-free version of ab270442. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ezrin with ab270442 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on biliary epithelial cells in rat liver (PMID: 23507543). The section was incubated with ab270442 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Immunofluorescent analysis of 100% methanol-fixed HeLa cells labeling Ezrin with ab270442 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    100% methanol fixation is recommended.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunoprecipitation - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunoprecipitation - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Ezrin was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab270442 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270442 at 1/1000 dilution (0.549 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a whole cell lysate 10 μg.
    Lane 2: ab270442 IP in Neuro-2a whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270442 in Neuro-2a whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Flow Cytometry - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Flow Cytometry - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Ezrin with ab270442 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunoprecipitation - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunoprecipitation - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Ezrin was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab270442 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270442 at 1/1000 dilution (0.549 μg/ml). VeriBlot for IP Detection Reagent (HRP )(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg.

    Lane 2: ab270442 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270442 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunoprecipitation - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunoprecipitation - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Ezrin was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab270442 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 270442 1/1000 dilution (0.549 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: C6 whole cell lysate 10 μg.
    Lane 2: ab270442 IP in C6 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270442 in C6 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 84 secs.

    The band of 55kDa is endogenously cleaved N-terminus form.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Ezrin with ab270442 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat spleen.The section was incubated with ab270442 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Ezrin with ab270442 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat kidney (PMID: 16522749). The section was incubated with ab270442 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ezrin with ab270442 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab270442 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Ezrin with ab270442 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID: 16522749). The section was incubated with ab270442 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Immunofluorescent analysis of 100% methanol-fixed Neuro-2a cells labeling Ezrin with ab270442 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in Neuro-2a cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    100% methanol fixation is recommended.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Flow Cytometry - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Flow Cytometry - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ezrin with ab270442 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Flow Cytometry - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Flow Cytometry - Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized C6 (Rat glial tumor glial cell) cells labeling Ezrin with ab270442 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270442).

  • Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)
    Anti-Ezrin antibody [EPR23353-55] - BSA and Azide free (ab270525)

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