Anti-EWI-2 antibody (ab74890)
Key features and details
- Rabbit polyclonal to EWI-2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EWI-2 antibody -
Description
Rabbit polyclonal to EWI-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human EWI-2 aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab74889) -
Positive control
- This antibody gave a positive signal in Human Brain Tissue Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).Target
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Function
May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. -
Tissue specificity
Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets. -
Sequence similarities
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type domains 3 and 4 are required for interaction with CD81.
The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 93185 Human
- Entrez Gene: 140559 Mouse
- Omim: 606644 Human
- SwissProt: Q969P0 Human
- SwissProt: Q8R366 Mouse
- Unigene: 332012 Human
- Unigene: 271717 Mouse
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Alternative names
- CD316 antibody
- CD316 antigen antibody
- CD81 partner 3 antibody
see all
Images
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Western blot - Anti-EWI-2 antibody (ab74890)Anti-EWI-2 antibody (ab74890) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesEWI-2 contains a number of potential glycosylation sites and a phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (1)
ab74890 has been referenced in 1 publication.
- Liang Y et al. Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J Biol Chem 289:32526-37 (2014). PubMed: 25261472
Images
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Western blot - Anti-EWI-2 antibody (ab74890)Anti-EWI-2 antibody (ab74890) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesEWI-2 contains a number of potential glycosylation sites and a phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
