Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERP27 antibody [EPR12503] (ab181172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12503] to ERP27
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ERP27 antibody [EPR12503]
    See all ERP27 primary antibodies

  • Description

    Rabbit monoclonal [EPR12503] to ERP27

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human, Mouse and Rat pancreas lysates, Human transitional cell carcinoma of bladder, BxP3-C cells and HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12503

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ERP27 antibody [EPR12503] (ab181172)
    Western blot - Anti-ERP27 antibody [EPR12503] (ab181172)
    Anti-ERP27 antibody [EPR12503] (ab181172) at 1/1000 dilution + Human pancreas at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ERP27 antibody [EPR12503] (ab181172)
    Immunocytochemistry/ Immunofluorescence - Anti-ERP27 antibody [EPR12503] (ab181172)

    Immunofluorescent staining of 4% paraformaldehyde fixed BxPC-3 cells, labeling ERP27 using ab181172 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.

  • Flow Cytometry - Anti-ERP27 antibody [EPR12503] (ab181172)
    Flow Cytometry - Anti-ERP27 antibody [EPR12503] (ab181172)

    Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling ERP27 using ab181172 at a 1/50 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERP27 antibody [EPR12503] (ab181172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERP27 antibody [EPR12503] (ab181172)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ERP27 using ab181172 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-ERP27 antibody [EPR12503] (ab181172)
    Western blot - Anti-ERP27 antibody [EPR12503] (ab181172)
    All lanes : Anti-C1orf163 antibody [EPR13779-15] (ab181176) at 1/1000 dilution

    Lane 1 : Rat pancreas
    Lane 2 : Mouse pancreas

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Anti-ERP27 antibody [EPR12503] (ab181172)
    Anti-ERP27 antibody [EPR12503] (ab181172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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