Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ERP27 antibody [EPR12503] (ab181172)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12503] to ERP27
Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-ERP27 antibody [EPR12503]
See all ERP27 primary antibodies
Description

Rabbit monoclonal [EPR12503] to ERP27
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human, Mouse and Rat pancreas lysates, Human transitional cell carcinoma of bladder, BxP3-C cells and HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12503
Isotype

IgG
Research areas

Western blot - Anti-ERP27 antibody [EPR12503] (ab181172)

Anti-ERP27 antibody [EPR12503] (ab181172) at 1/1000 dilution + Human pancreas at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ERP27 antibody [EPR12503] (ab181172)

Immunofluorescent staining of 4% paraformaldehyde fixed BxPC-3 cells, labeling ERP27 using ab181172 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
Flow Cytometry - Anti-ERP27 antibody [EPR12503] (ab181172)

Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling ERP27 using ab181172 at a 1/50 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERP27 antibody [EPR12503] (ab181172)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ERP27 using ab181172 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-ERP27 antibody [EPR12503] (ab181172)

All lanes : Anti-C1orf163 antibody [EPR13779-15] (ab181176) at 1/1000 dilution

Lane 1 : Rat pancreas
Lane 2 : Mouse pancreas

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugated at 1/1000 dilution

Predicted band size: 30 kDa
Anti-ERP27 antibody [EPR12503] (ab181172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com