Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERGIC3 antibody [EPR8141] (ab129179)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8141] to ERGIC3
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ERGIC3 antibody [EPR8141]
    See all ERGIC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8141] to ERGIC3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ERGIC3 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, MCF7, 293T, K562 and HeLa cell lysates. IHC-P: Human brain tissue. ICC: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 5.36 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8141

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)
    Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)
    All lanes : Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ERGIC3 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab129179 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab129179 Anti-ERGIC3 antibody [EPR8141] was shown to specifically react with ERGIC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266489 (knockout cell lysate ab257941) was used. Wild-type and ERGIC3 knockout samples were subjected to SDS-PAGE. ab129179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (ab129179)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (ab129179)

    ab129179, at 1/100, staining ERGIC3 in paraffin embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (ab129179)
    Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (ab129179)

    ab129179, at 1/250, staining ERGIC3 in HeLa cells by Immunofluorescence.

  • Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)
    Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)
    All lanes : Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (ab129179)
    OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (ab129179)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ERGIC3 antibody [EPR8141] (ab129179)
    Anti-ERGIC3 antibody [EPR8141] (ab129179)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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