Anti-Enkephalin/ENK antibody (ab85798)
Key features and details
- Rabbit polyclonal to Enkephalin/ENK
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Enkephalin/ENK antibody
See all Enkephalin/ENK primary antibodies -
Description
Rabbit polyclonal to Enkephalin/ENK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Enkephalin/ENK aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102738) -
Positive control
- This antibody gave a positive signal in Human testis tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications MouseRatApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).Target
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Function
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. -
Sequence similarities
Belongs to the opioid neuropeptide precursor family. -
Post-translational
modificationsThe N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5179 Human
- Entrez Gene: 18619 Mouse
- Entrez Gene: 29237 Rat
- Omim: 131330 Human
- SwissProt: P01210 Human
- SwissProt: P22005 Mouse
- SwissProt: P04094 Rat
- Unigene: 339831 Human
see all -
Alternative names
- ENK antibody
- Enkephalin A antibody
- Enkephalin antibody
see all
Images
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Western blot - Anti-Enkephalin/ENK antibody (ab85798)Anti-Enkephalin/ENK antibody (ab85798) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThe band observed at 28 kDa could potentially be a cleaved form of Enkephalin due to the presence of a 24 amino acid signal peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Enkephalin/ENK antibody (ab85798)ICC/IF image of ab85978 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85798, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
References (1)
ab85798 has been referenced in 1 publication.
- Bulc M et al. Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes. J Diabetes Res 2018:4735659 (2018). PubMed: 30140706
Images
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Western blot - Anti-Enkephalin/ENK antibody (ab85798)Anti-Enkephalin/ENK antibody (ab85798) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThe band observed at 28 kDa could potentially be a cleaved form of Enkephalin due to the presence of a 24 amino acid signal peptide.
-
Immunocytochemistry/ Immunofluorescence - Anti-Enkephalin/ENK antibody (ab85798)ICC/IF image of ab85978 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85798, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
