Anti-Endothelin 1 antibody (ab113697)
Key features and details
- Rabbit polyclonal to Endothelin 1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Endothelin 1 antibody
See all Endothelin 1 primary antibodies -
Description
Rabbit polyclonal to Endothelin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human Lung and Placenta tissue lysates. It also gave a positive signal in FFPE human placenta tissue sections and JEG3 cell line in IF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB 1/250. Detects a band of approximately 15 kDa (predicted molecular weight: 24 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Endothelins are endothelium-derived vasoconstrictor peptides. -
Tissue specificity
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. -
Sequence similarities
Belongs to the endothelin/sarafotoxin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1906 Human
- Entrez Gene: 13614 Mouse
- Omim: 131240 Human
- SwissProt: P05305 Human
- SwissProt: P22387 Mouse
- Unigene: 511899 Human
- Unigene: 713645 Human
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Alternative names
- ARCND3 antibody
- Big endothelin-1 antibody
- EDN1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody (ab113697)IHC image of Endothelin 1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113697, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Endothelin 1 antibody (ab113697)All lanes : Anti-Endothelin 1 antibody (ab113697) at 1/250 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 15 kDa could potentially be a cleaved form of Endothelin 1 due to the presence of both a signal and pro-peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody (ab113697)ab113697 stained in JEG3 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113697 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature
Protocols
Datasheets and documents
References (3)
ab113697 has been referenced in 3 publications.
- Xie M et al. MicroRNA-1 acts as a tumor suppressor microRNA by inhibiting angiogenesis-related growth factors in human gastric cancer. Gastric Cancer 21:41-54 (2018). WB . PubMed: 28493075
- Calhan OY & Seyrantepe V Mice with Catalytically Inactive Cathepsin A Display Neurobehavioral Alterations. Behav Neurol 2017:4261873 (2017). IHC-Fr ; Mouse . PubMed: 28133419
- Daehn I et al. Endothelial mitochondrial oxidative stress determines podocyte depletion in segmental glomerulosclerosis. J Clin Invest 124:1608-21 (2014). IHC-P . PubMed: 24590287
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody (ab113697)IHC image of Endothelin 1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113697, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Endothelin 1 antibody (ab113697)All lanes : Anti-Endothelin 1 antibody (ab113697) at 1/250 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 15 kDa could potentially be a cleaved form of Endothelin 1 due to the presence of both a signal and pro-peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody (ab113697)
ab113697 stained in JEG3 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113697 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature
