Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15672] to ENDOGL1/ENGL - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free
    See all ENDOGL1/ENGL primary antibodies

  • Description

    Rabbit monoclonal [EPR15672] to ENDOGL1/ENGL - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251450 is the carrier-free version of ab206300 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251450 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ENDOGL1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
    Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
    All lanes : Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300) at 1/1000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 3 minutes


    This data was developed using ab206300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
    Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
    All lanes : Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 3 minutes


    This data was developed using ab206300, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
    Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
    This data was developed using ab206300, the same antibody clone in a different buffer formulation.ENDOGL1/ENGL was immunoprecipitated from 1mg of HUVEC (Human umbilical vein endothelial cell line) whole cell lysate with ab206300 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206300 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HUVEC whole cell lysate 10ug (Input). Lane 2: ab206300 IP in HUVEC whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206300 in HUVEC whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
    Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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