Anti-EMX2 antibody (ab94713)
Key features and details
- Rabbit polyclonal to EMX2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-EMX2 antibody
See all EMX2 primary antibodies -
Description
Rabbit polyclonal to EMX2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Chicken, Cow, Dog, Human, Pig, Zebrafish -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and hippocampus tissue lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...
Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab94713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Target
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Function
Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. -
Tissue specificity
Cerebral cortex. -
Involvement in disease
Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid. -
Sequence similarities
Belongs to the EMX homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 523601 Cow
- Entrez Gene: 2018 Human
- Entrez Gene: 13797 Mouse
- Entrez Gene: 499380 Rat
- Omim: 600035 Human
- SwissProt: Q17R00 Cow
- SwissProt: Q04743 Human
- SwissProt: Q04744 Mouse
see all -
Alternative names
- Empty spiracles homeobox 2 antibody
- Empty spiracles homolog 2 (Drosophila) antibody
- Empty spiracles homolog 2 antibody
see all
Images
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All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml
Lane 1 : Mouse Hippocampus Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Additional bands at: 260 kDa (possible non-specific binding), 76 kDa (possible non-specific binding)
Exposure time: 16 minutes
Protocols
Datasheets and documents
References (0)
ab94713 has not yet been referenced specifically in any publications.
Images
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All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml
Lane 1 : Mouse Hippocampus Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Additional bands at: 260 kDa (possible non-specific binding), 76 kDa (possible non-specific binding)
Exposure time: 16 minutes