Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)
![Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)](https://www.abcam.com/ps/products/254/ab254293/Images/ab254293-384762-anti-emr1-antibody-epr2322594-western-blot-human-colon-human-stomach-lysates.jpg "Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23225-94] to EMR1/ADGRE1
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-EMR1/ADGRE1 antibody [EPR23225-94]
See all EMR1/ADGRE1 primary antibodies -
Description
Rabbit monoclonal [EPR23225-94] to EMR1/ADGRE1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human spleen, colon and stomach lysates. IHC-P: Human gastric cancer and colon tissues. ICC/IF: HEK-293 cells transfected with myc-tagged EMR1/ADGRE1 expression vector. Flow Cyt: HEK-293 cells transfected with myc-tagged EMR1/ADGRE1 expression vector.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23225-94 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)All lanes : Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293) at 1/1000 dilution
Lane 1 : Human colon tissue lysate
Lane 2 : Human stomach tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 70 seconds.
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![Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)](https://www.abcam.com/ps/products/254/ab254293/Images/ab254293-384760-anti-emr1-antibody-epr2322594-western-blot-human-spleen-human-brain-lysates.jpg)
Western blot - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)All lanes : Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293) at 1/1000 dilution
Lane 1 : Human spleen tissue lysate
Lane 2 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Negative control: Human brain (PMID:17823986).
Exposure time: 3 minutes.
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![Flow Cytometry - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)](https://www.abcam.com/ps/products/254/ab254293/Images/ab254293-5-anti-emr1-antibody-epr2322594-flow-cytometry-293t-transfected-with-myc-tagged-emr1-construct.jpg)
Flow Cytometry - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HEK-293T (transfected with myc tagged EMR1/ADGRE1 construct) cells labelling EMR1/ADGRE1 with ab254293 at 1/500 (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Cells were surface stained with isotype control (ab172730) or ab254293. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.
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![Immunocytochemistry/ Immunofluorescence - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)](https://www.abcam.com/ps/products/254/ab254293/Images/ab254293-4-anti-emr1-antibody-epr2322594-immunocytochemistry-hek293.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 cells labelling EMR1/ADGRE1 with ab254293 at 1/100 dilution, followed by antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293 cell line transfected with myc-tagged EMR1/ADGRE1 expression vector. 2233S Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is at 1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)](https://www.abcam.com/ps/products/254/ab254293/Images/ab254293-2-anti-emr1-antibody-epr2322594-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EMR1/ADGRE1 with ab254293 at 1/16000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in eosinophils of human colon (PMID:17823986).The section was incubated with ab254293 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)](https://www.abcam.com/ps/products/254/ab254293/Images/ab254293-1-anti-emr1-antibody-epr2322594-immunohistochemistry-human-gastric-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling EMR1/ADGRE1 with ab254293 at 1/16000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in eosinophils of human gastric cancer (PMID:17823986).The section was incubated with ab254293 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)](https://www.abcam.com/ps/products/254/ab254293/Images/ab254293-8-benefits-of-recombinant-antibodies.png)
Anti-EMR1/ADGRE1 antibody [EPR23225-94] (ab254293)