Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13374-2] to Elav-type RNA-binding protein ETR3
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2]
    See all Elav-type RNA-binding protein ETR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13374-2] to Elav-type RNA-binding protein ETR3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, K562, HeLa, MCF7, C6 and NIH 3T3 lysates; Human brain tissue; rat lung tissue; MCF7 and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    ab186430 staining Elav-type RNA-binding protein ETR3 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

  • Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Flow Cytometry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Flow Cytometry - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/270 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

    Western blot analysis of Elav-type RNA-binding protein ETR3 in HeLa cell lysate immunoprecipitated with ab186430 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)
    Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

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