Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)

Key features and details

  • Mouse monoclonal [1B6A3] to EKLF / KLF1
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-EKLF / KLF1 antibody [1B6A3]
    See all EKLF / KLF1 primary antibodies

  • Description

    Mouse monoclonal [1B6A3] to EKLF / KLF1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human EKLF/ KLF1 aa 208-362. Expressed in E. Coli.
    Sequence:

    PAPQYQGHFQLFRGLQGPAPGPATSPSFLSCLGPGTVGTGLGGTAEDPGV IAETAPSKRGRRSWARKRQAAHTCAHPGCGKSYTKSSHLKAHLRTHTGEK PYACTWEGCGWRFARSDELTRHYRKHTGQRPFRCQLCPRAFSRSDHLALH MKRHL


    Database link: Q13351
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human EKLF / KLF1 recombinant protein; HeLa cells; Human cervical and rectum cancer tissues

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR252532-8 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
    Western blot - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
    Anti-EKLF / KLF1 antibody [1B6A3] (ab175372) at 1/500 dilution + Human EKLF / KLF1 recombinant protein

    Predicted band size: 38 kDa



    Expected MW is 42.6 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)

    Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling EKLF / KLF1 using ab175372 at 1/200 dilution, followed by DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)

    Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling EKLF / KLF1 using ab175372 at 1/200 dilution, followed by DAB staining.

  • Flow Cytometry - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
    Flow Cytometry - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)

    Flow cytometric analysis of HeLa cells labeling EKLF / KLF1 using ab175372 at 1/200 dilution (green) and negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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