Anti-eIF4E antibody (ab72116)
Key features and details
- Rabbit polyclonal to eIF4E
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4E antibody
See all eIF4E primary antibodies -
Description
Rabbit polyclonal to eIF4E -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to a region between residue 1 and 50 of human eIF4E (NP_001959.1).
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Positive control
- HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab72116 was affinity purified using an epitope specific to eIF4E immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4E antibody (ab72116)All lanes : Anti-eIF4E antibody (ab72116) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK-1 cell lysate
Lane 5 : NIH3T3 cell lysate
Predicted band size: 25 kDaDetection: Chemiluminescence with an exposure time of 10 seconds.
Previous -
Immunoprecipitation - Anti-eIF4E antibody (ab72116)Detection of eIF4E by Western Blot of Immunprecipitate.
anti-eIF4E at 1µg/ml staining eIF4E in HeLa whole cell lysate immunoprecipitated using ab72116 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
