Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF4A1 antibody [EPR14506] (ab185946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14506] to eIF4A1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF4A1 antibody [EPR14506]
    See all eIF4A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14506] to eIF4A1

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 93% homology with eIF4A2. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, 293, HeLa, MCF7, mouse Raw264.7, mouse NIH 3T3, rat C6 and rat Pc12 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    All lanes : Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    All lanes : Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/1000 dilution

    Lane 1 : rat C6 cell lysate
    Lane 2 : mouse Raw264.7 cell lysate
    Lane 3 : rat PC12 cell lysate
    Lane 4 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (ab185946)
    Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (ab185946)

    Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling eIF4A1 using ab185946 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-eIF4A1 antibody [EPR14506] (ab185946)
    Anti-eIF4A1 antibody [EPR14506] (ab185946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-eIF4A1 antibody [EPR14506] (ab185946)

© 2021 Astana Biomed Group LLP. All rights reserved.