Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 110 563 ₸

Order now and get it on Wednesday March 10, 2021

Anti-eIF4A1 antibody [EPR14506] (ab185946)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14506] to eIF4A1
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-eIF4A1 antibody [EPR14506]
See all eIF4A1 primary antibodies
Description

Rabbit monoclonal [EPR14506] to eIF4A1
Host species

Rabbit
Specificity

The immunogen used for this product shares 93% homology with eIF4A2. Cross-reactivity with this protein has not been confirmed experimentally.
Tested Applications & Species

Application Species
IP
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat, 293, HeLa, MCF7, mouse Raw264.7, mouse NIH 3T3, rat C6 and rat Pc12 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14506
Isotype

IgG
Research areas

Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)

All lanes : Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa
Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946)

All lanes : Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/1000 dilution

Lane 1 : rat C6 cell lysate
Lane 2 : mouse Raw264.7 cell lysate
Lane 3 : rat PC12 cell lysate
Lane 4 : mouse NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa
Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (ab185946)

Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling eIF4A1 using ab185946 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
Anti-eIF4A1 antibody [EPR14506] (ab185946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com