Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)
![Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)](https://www.abcam.com/ps/products/263/ab263951/Images/ab263951-355847-ab2421481antieif2s1antibodyepr2309850immunohistochemistrybreasthuman.jpg "Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23098-50] to EIF2S1 - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free
See all EIF2S1 primary antibodies -
Description
Rabbit monoclonal [EPR23098-50] to EIF2S1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, HepG2, HeLa, RAW264.7, C6, Human spleen, Rat kidney, Rat spleen and Mouse heart lysates. IHC-P: Human breast, Mouse kidney and Rat cerebrum tissues. IP: MCF7 cells.
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General notes
ab263951 is the carrier-free version of ab242148. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab263951 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23098-50 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242148).
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![Immunoprecipitation - Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)](https://www.abcam.com/ps/products/263/ab263951/Images/ab263951-355850-ab2421484antieif2s1antibodyepr2309850immunoprecipitationmcf7human.jpg)
Immunoprecipitation - Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)EIF2S1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug with ab242148 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242148 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab242148 IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242148 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds
This blot was developed using a higher sensitivity ECL substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242148).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)](https://www.abcam.com/ps/products/263/ab263951/Images/ab263951-355849-ab2421483antieif2s1antibodyepr2309850immunohistochemistrycerebrumrat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242148).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)](https://www.abcam.com/ps/products/263/ab263951/Images/ab263951-355848-ab2421482antieif2s1antibodyepr2309850immunohistochemistrykidneymouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining mouse kidney. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242148).
-
![Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)](https://www.abcam.com/ps/products/263/ab263951/Images/ab263951-5-benefits-of-recombinant-antibodies.png)
Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (ab263951)
