Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 107 212 ₸

Order now and get it on Tuesday March 09, 2021

Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23098-50] to EIF2S1
Suitable for: IP, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-EIF2S1 antibody [EPR23098-50]
See all EIF2S1 primary antibodies
Description

Rabbit monoclonal [EPR23098-50] to EIF2S1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, HepG2, HeLa, RAW264.7, C6, Human spleen, Rat kidney, Rat spleen and Mouse heart lysates. IHC-P: Human breast, Mouse kidney and Rat cerebrum tissues. IP: MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23098-50
Isotype

IgG
Research areas

Western blot - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

All lanes : Anti-EIF2S1 antibody [EPR23098-50] (ab242148) at 1/1000 dilution

Lane 1 : Human spleen tissue lysate
Lane 2 : Rat kidney tissue lysate
Lane 3 : Rat spleen tissue lysate
Lane 4 : Mouse heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Lane 1: 48 seconds Lanes 2-3: 15 seconds Lane 4: 59 seconds
Western blot - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

All lanes : Anti-EIF2S1 antibody [EPR23098-50] (ab242148) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: Lanes 1/3-5: 10 seconds Lane 2: 81 seconds
Immunoprecipitation - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

EIF2S1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug with ab242148 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242148 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

Lane 2: ab242148 IP in MCF7 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242148 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 50 seconds

This blot was developed using a higher sensitivity ECL substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining mouse kidney. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins
Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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