Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23098-50] to EIF2S1
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EIF2S1 antibody [EPR23098-50]
    See all EIF2S1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23098-50] to EIF2S1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, HepG2, HeLa, RAW264.7, C6, Human spleen, Rat kidney, Rat spleen and Mouse heart lysates. IHC-P: Human breast, Mouse kidney and Rat cerebrum tissues. IP: MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    Western blot - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    All lanes : Anti-EIF2S1 antibody [EPR23098-50] (ab242148) at 1/1000 dilution

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Rat kidney tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : Mouse heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.  

    Exposure time: Lane 1: 48 seconds Lanes 2-3: 15 seconds Lane 4: 59 seconds

  • Western blot - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    Western blot - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    All lanes : Anti-EIF2S1 antibody [EPR23098-50] (ab242148) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST   Exposure time: Lanes 1/3-5: 10 seconds Lane 2: 81 seconds

  • Immunoprecipitation - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    Immunoprecipitation - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

    EIF2S1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug with ab242148 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242148 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab242148 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242148 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 50 seconds

    This blot was developed using a higher sensitivity ECL substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling EIF2S1 with ab242148 at 1:4000 dilution (0.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining mouse kidney. The section was incubated with ab242148 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins

  • Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
    Anti-EIF2S1 antibody [EPR23098-50] (ab242148)

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