Anti-EHHADH antibody (ab123490)
Key features and details
- Rabbit polyclonal to EHHADH
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EHHADH antibody
See all EHHADH primary antibodies -
Description
Rabbit polyclonal to EHHADH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 673-723 of Human EHHADH using the numbering given in entry NP_001957.2.
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Positive control
- Whole cell lysate from 293T, Jurkat, HeLa
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EHHADH antibody (ab123490)All lanes : Anti-EHHADH antibody (ab123490) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-EHHADH antibody (ab123490)Lane 1: Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of EHHADH.
Lane 2: Detection of Human EHHADH by Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123490 at 6 µg/mg lysate for IP.
Lane 3: Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab123490 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
