Anti-EHD2 antibody (ab23935)
Key features and details
- Goat polyclonal to EHD2
- Suitable for: Flow Cyt, ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EHD2 antibody
See all EHD2 primary antibodies -
Description
Goat polyclonal to EHD2 -
Host species
Goat -
Specificity
This antibody is expected to recognise EHD1 protein as well as EHD2. -
Tested applications
Suitable for: Flow Cyt, ICC, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Dog
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Immunogen
Synthetic peptide:
C-RLVPPSKRRHKGSA
, corresponding to C terminal amino acids 529 - 542 of Human EHD2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-EHD2 antibody (ab23935)
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation with ab23935 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Flow Cytometry - Anti-EHD2 antibody (ab23935)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation ab23935 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-EHD2 antibody (ab23935)Anti-EHD2 antibody (ab23935) at 0.3 µg/ml + Human lung lysate at 35 µg
Predicted band size: 61 kDa
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Immunocytochemistry - Anti-EHD2 antibody (ab23935)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Western blot - Anti-EHD2 antibody (ab23935)Lane 1 : Anti-EHD2 antibody (ab23935) at 0.1 µg/ml
Lane 2 : Anti-EHD2 antibody (ab23935) at 0.3 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 61 kDa
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Immunocytochemistry - Anti-EHD2 antibody (ab23935)ICC/IF image of ab23935 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23935, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
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Western blot - Anti-EHD2 antibody (ab23935)Anti-EHD2 antibody (ab23935) at 0.003 µg/ml + Human Heart lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 61 kDa
Observed band size: 61 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Western blot - Anti-EHD2 antibody (ab23935)Anti-EHD2 antibody (ab23935) at 0.1 µg/ml + Human Placenta lysate in RIPA buffer at 35 µg
Predicted band size: 61 kDa
