Anti-EGFR (phospho Y1045) antibody - C-terminal (ab227022)
Key features and details
- Rabbit polyclonal to EGFR (phospho Y1045) - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EGFR (phospho Y1045) antibody - C-terminal
See all EGFR primary antibodies -
Description
Rabbit polyclonal to EGFR (phospho Y1045) - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human EGFR (C terminal) (phospho Y1045). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P00533 -
Positive control
- WB: Serum starved A431 treated with EGF, whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho Y1045) antibody - C-terminal (ab227022)All lanes : Anti-EGFR (phospho Y1045) antibody - C-terminal (ab227022) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line), starved for 16 hours, whole cell extract
Lane 2 : A431, starved for 16 hours and treated with 100 ng/ml EGF for 5 minutes, whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 134 kDa7.5% SDS-PAGE gel.
