Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)
![Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)](https://www.abcam.com/ps/products/192/ab192263/Images/ab192263-229984-anti-egfr-mutated-l858-r-antibody-epr15348-western-blot.jpg "Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15348] to EGFR (mutated L858R)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-EGFR (mutated L858R) antibody [EPR15348]
See all EGFR primary antibodies -
Description
Rabbit monoclonal [EPR15348] to EGFR (mutated L858R) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: H1975 cell lysates. IHC-P: Human lung carcinoma tissue. ICC/IF: HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15348 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)All lanes : Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263) at 1/1000 dilution
Lane 1 : H1975 cell lysate
Lane 2 : NCI-H460 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 134 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)](https://www.abcam.com/ps/products/192/ab192263/Images/ab192263-253672-anti-egfr-mutated-l858-r-antibody-epr15348-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling EGFR with ab192263 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).
The negative controls are as follows;
1. ab192263 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor® 594) at 1/500 dilution.
2. ab192263 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor® 594) at 1/500 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)](https://www.abcam.com/ps/products/192/ab192263/Images/ab192263-229985-anti-egfr-mutated-l858-r-antibody-epr15348-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling EGFR with ab192263 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. The negative control also shown. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)](https://www.abcam.com/ps/products/192/ab192263/Images/ab192263-5-benefits-of-recombinant-antibodies.png)
Anti-EGFR (mutated L858R) antibody [EPR15348] (ab192263)
